Composite

Part:BBa_K648128

Designed by: Lauren Rossi   Group: iGEM11_Penn_State   (2011-10-28)


Or tester with Cro

This is a test circuit comprised of GFP, mCherry, operating region (Or) of lambda phage, pBAD, and Cro with RBS B0034. When the circuit is induced, the Cro is expressed. Cro will bind to the binding sites of the operating region. The GFP used is the reverse sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1943
    Illegal BamHI site found at 1789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


[edit]
Categories
Parameters
n/aOr tester with Cro