Composite

Part:BBa_K648030

Designed by: James Coletta, Anisha Katyal, Kristen Salavo   Group: iGEM11_Penn_State   (2011-07-25)


Or with RBS B0034 and Mcherry

The Operating region of the lambda phage switch system with Mcherry on a strong RBS attached after the pr promoter of Or. We used this part in our project to test the pr promoter in Or. Since Mcherry has RFP, one can measure the translation rate of Mcherry. Theoretically you could attach C1 to the prm promoter of Or in order to test its repression and no fluoresence should be observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aOr with RBS B0034 and Mcherry