Coding
Part:BBa_K648028
Designed by: James Coletta, Anisha Katyal, Kristen Salavo, Lauren Rossi Group: iGEM11_Penn_State (2011-07-25)
Cro, Lamda Repressor which activates the lytic cycle
Cro is produced by the pr promoter in the lambda phage system. Normally C1 is repressing the pr promoter. However, when the lytic switch is thrown and RecA cleaves the C1 dimer the pr promoter is unrepressed. This results in the production of cro which in turn represses the prm promoter which halts the transcription of C1 repressor. In our project we used Cro to assemble our lambda system.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |