Reporter

Part:BBa_K648010

Designed by: Jim Rose   Group: iGEM11_Penn_State   (2011-07-04)


Lac-Inducible C230 Reporter

This is a composite part comprised of the Plac promoter (BBa_R0010) and the Xyle gene with RBS (BBa_J33204). The Xyle gene encodes for the enzyme catechol-2,3-dioxygenase (metapyrocatechase), which converts catechol to the bright yellow product 2-hydroxy-cis,cis-muconic semialdehyde. For more information see part J33204.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 545
    Illegal NgoMIV site found at 717
    Illegal AgeI site found at 1068
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None