Translational_Unit
AT-Bph
Part:BBa_K646001
Designed by: David Dubow Group: iGEM11_Macquarie_Australia (2011-09-29)
Agrobacterium tumefaciens Bacteriophotochrome (Rhizobium radiobacter)
The Agrobacterium tumefaciens bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. There is a ribosome binding site six bases upstream from the ATG start codon. Sequence from National Center for Biotechnology.
This part was made using BBa_K497004 and PCR and contains a RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1867
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1196
Illegal NgoMIV site found at 1694
Illegal AgeI site found at 1759 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |