Composite

Part:BBa_K643001

Designed by: Alison Acevedo, Peter (Renxuan) Liu   Group: iGEM11_Columbia-Cooper   (2011-09-27)


Blue Light Promoter controlling Chloramphenicol Resistance

Blue Light Promoter (BLP) controlling Chloramphenicol Resistance (Chlor) on pSB1A3 vector BBa_P1004 (Chlor) BBa_K238013 (BLP)

Reporter ycgF responds to blue light changing its conformation and dimerizing with ycgE molecules. Because of the dimerization, ycgF releases from the promoter region. Consequently, ycgF no longer represses gene transcription. Downstream, the gene for chloramphenicol resistance is now accessible to transcription enzymes. Thus, blue light initializes chloramphenicol resistance.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aBlue Light Promoter controlling Chloramphenicol Resistance