Composite

Part:BBa_K641009

Designed by: Spencer Scott   Group: iGEM11_Berkeley   (2011-09-01)

P_rffGH.rbs.ToxR-Nhe1.{gsgsgs}{LambdaRep}

This composite part constitutively expresses a ToxR-LambdaRep construct. This ToxR construct will dimerize at a high affinity, because of Lambda Rep's dimerizing properties, making the cytoplasmic ToxR dimer an active transcription factor for the ctx Promoter. Thus, If this composite part is combined with a Pctx-GFP reporter there will be a high expression of GFP.

The figures below show the fluorescence difference between:

  1. Plain MC1061 Cells
  2. The Bss52 Reporter by itself
  3. MC1061 cells with a Constitutive GFP plasmid
  4. The Bss52 Reporter with a ToxR-LambdaRep that causes constitutive Pctx activation

LambdaREpCharacterizationPage.jpg

This is available in .pdf format here: File:LambdaREpCharacterizationPage(2).pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1008
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None