Part:BBa_K641008

{Pctx}{rbs.ffGFP}

This part is a general reporter for any ToxR system. When ToxR dimerizes it binds to Pctx and activates Transcription of genes downstream of Pctx. By putting ffGFP downstream of Pctx the strength of the ToxR construct dimerization is reported by a corresponding strength of GFP expression. The figures below show the fluorescence difference between:

1. Plain MC1061 Cells
2. The Bss52 Reporter by itself
3. MC1061 cells with a Constitutive GFP plasmid
4. The Bss52 Reporter with a ToxR-MukF variant that causes constitutive Pctx activation
5. The Bss52 Reporter with a ToxR-MukF variant that is broken (No/little Pctx activation)

This is available in .pdf format here: File:Bss52CharacterizationPage(3).pdf

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

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