Part:BBa_K635001
Composite coding region for PDGF-β in yeast
This part contains a platelet derived growth factor (PDGF) sequence modified for effective expression within a yeast chassis. The part contains the cDNA sequence for PDGF codon-optimized for yeast expression. It also contains the biobrick kozak sequence [BBa_J63003]. Preceding the kozak sequence is a random buffer sequence complementary to an siRNA inhibitor designed for modular inhibition of translation in yeast. The siRNA sequence ([BBa_K635000]) is reverse complementary to both the kozak sequence and the random buffer. This idea was derived from the 2010 Stanford team's modular siRNA regulation in E.coli. Flanking the cDNA sequence is also a poly-A tail necessary for expression in eukaryotic hosts, such as yeast, to prevent degradation of the mRNA fragment. Following the poly-A tail is the biobrick [BBa_K392003] yeast transcriptional terminator. Together these preparatory components allow for expression of PDGF within a yeast chassis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 135
Illegal BglII site found at 288
Illegal BglII site found at 351
Illegal BglII site found at 474 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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