Part:BBa_K632003

Ho1-PcyA-cph8, regulatory system

Endogenous heme is converted into biliverdin by Phycocyanobilin:ferredoxin oxidoreductase (pcyA). The biliverdin is then converted by Heme oxigenase (ho1) to phycocyanobilin (PCB), which associates with a chimeric light receptor, cph8 which is the fusion product of Cph1 and Envz-OmpR, obtained from BBa_I15010 (https://parts.igem.org/wiki/index.php?title=Part:BBa_I15010). The PCB and cph8 complex phosphorylates the regulatory protein OmpR, which can then activate the OmpC promoter (pompC) to activate the expression of a desired gene. Stimulation of the cph8 component with red light (~660 nm) inhibits autophosporylation of the receptor which can be used to produced a differential output of a desired gene.

[http://2011.igem.org/File:Umn_coliroid_silicatein_system.png Example Diagram of Part Function]

References:

Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, and Voigt CA. Synthetic biology: engineering *Escherichia coli to see light. Nature 2005 Nov 24; 438(7067) 441-2. doi:10.1038/nature04405 pmid:16306980.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2257
  Illegal BglII site found at 3038
  Illegal XhoI site found at 364
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2591
  
• 1000
  COMPATIBLE WITH RFC[1000]