Part:BBa_K631031
ODR7:OX1R:UNC-54 (for use in C. elegans)
Composite part designed to modify chemotaxis of C. elegans.
Characterization
Part was characterized through the use of a chemotaxis assay.
Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. Target chemical used included naphthalene, pond tailings sample, bitumen sample, and 4-chlorobenzoic acid.
Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. Any worm contained in the circular zone approximately 0.5mm around centre of agar plate was not counted as either in a control or target quadrant. An example agar plate is shown below.
To determine quantitative measurement, the chemotactic index was determined. The formula for this index is shown below:
Result
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2082
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1308
Illegal BglII site found at 2147
Illegal BglII site found at 2219 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2712
Illegal SapI site found at 1845
Illegal SapI site found at 2100
None |