Composite

Part:BBa_K631031

Designed by: Allister Smith   Group: iGEM11_Queens_Canada   (2011-09-15)

ODR7:OX1R:UNC-54 (for use in C. elegans)

Composite part designed to modify chemotaxis of C. elegans.

Characterization
Part was characterized through the use of a chemotaxis assay. Agar plates were divided into four quadrants, 2 areas corresponding to 'target' and 2 'control' sections. In the target sections, differing concentrations of phenol were placed while control section used distilled water. Sodium azide was used as a paralytic in each quadrant to 'freeze' worms once they chemotaxed to that quadrant. Target chemical used included naphthalene, pond tailings sample, bitumen sample, and 4-chlorobenzoic acid.

Worms were placed at the centre of the agar plate (inside circle) and were allowed to chemotax for one hour. After one hour, worms were counted in each quadrant. Any worm contained in the circular zone approximately 0.5mm around centre of agar plate was not counted as either in a control or target quadrant. An example agar plate is shown below.

Chemotaxis.png


To determine quantitative measurement, the chemotactic index was determined. The formula for this index is shown below:

Queens CanadaEquation.png



Result

Ox1rptfinal.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1308
    Illegal BglII site found at 2147
    Illegal BglII site found at 2219
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2712
    Illegal SapI site found at 1845
    Illegal SapI site found at 2100


[edit]
Categories
Parameters
None