Part:BBa_K629011

recNp-trkD-eGFP

Background

recN-trkD-eGFP.In the project, this part is consist of recNp, trkD. When E. Coli approaches radioactive elements, the intensity of radiation is much more than distant areas. After the intensity is higher than the threshold of recNp, the high expression of TrkD would be started. As a result, E. Coli would absorb cesium-137 around it. At the same time, eGFP will express and show the location of e.coli.

Expression in E.coli

The expression of part TrkD-GFP on plasmid pUC18 in E.coli.

Figure1 trkD-GFP-pUC18-control 1

Figure2 trkD-GFP-pUC18-experiment 1

Figure3 trkD-GFP-pUC18-control 2

Figure4 trkd-GFP-pUC18-experiment 2

Microplate Reader result of part recN-GFP on plasmid pET28

Figure5 Fluorescence intensity of recN-GFP-pET28a-BL21 in all the five experiments

Discussion

recN-GFP-pET28a-BL21 has a platform instead of a trough. Then a crest after that. We guess that the threshold of recN promoter is higher than recA promoter is the reason. The crest caused by the recN promoter in the plasmid overlappes with the trough, which creates a platform as we see. As the why the the rec promoter of the plasmid will be damaged earlier than that in the genome, we guess the different position of the two promoters is the reason.

However, the difference between all the data is not convincing enough, which may lead to another explanation: all the tendency of the data is caused by system error, and yet, which we haven’t found out.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 378
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1453