Part:BBa_K624054

mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

This part is composed of BBa_K624045 and BBa_K624055, both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624045 in the downstream of BBa_K624055. If so, unwanted leakiness may happen. The construct was inserted into pYMB(BBa_K624027) and then was electropored into AMB-1(BBa_K624032).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 842
Illegal PstI site found at 1622
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 842
Illegal PstI site found at 1622
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 842
Illegal PstI site found at 1622
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 842
Illegal PstI site found at 1622
Illegal NgoMIV site found at 2599
Illegal AgeI site found at 681
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 4262