Regulatory

Part:BBa_K624053

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

mcherry+minC+RBS+tetO+pT7 + pT7+RBS+tetR+GFP

mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

This part is composed of BBa_K624041 and BBa_K624002, both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624041 in the downstream of BBa_K624002. If so, unwanted leakiness may happen. The construct was inserted in pUC19 and then was transformed into E. coli(BL21).

E.coli minC NYMU.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 803
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 803
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 803
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 803
    Illegal AgeI site found at 681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2898


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Categories
Parameters
None