Coding

Part:BBa_K624050

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

RBS(trunc.) + minC + RBS(trunc.) + mcherry

RBS(trunc.) + minC + RBS(trunc.) + mcherry

All of the ribosome binding sites are RBS from Pmsp3 with 6 bps truncated(BBa_K624013).

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 239
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None