Coding

Part:BBa_K624049

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

RBS(Pmsp3) + minC + RBS(Pmsp3) +mcherry

RBS(Pmsp3) + minC + mcherry

RBS(Pmsp3) is the RBS site from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 248
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None