Generator

Part:BBa_K624045

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taiwan   (2011-10-05)

Pmsp1(tetO) + RBS(Pmsp3) + minC + RBS(Pmsp3) + mcherry

Pmsp1(tetO) + RBS(Pmsp3) + minC + RBS(Pmsp3) + mcherry

Pmsp1(tetO) (BBa_K624046) is composed of Pmsp1, the second strongest promoter on Magnetospirillum magneticum AMB-1, with tetO between -10 and -35 boxes.

MinC (BBa_K624033) is known as a cell division inhibitor.

All of the ribosome binding sites are from Pmsp3, the strongest promoter of AMB-1.(BBa_K624012)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None