Primer

Part:BBa_K624035

Designed by: Yi-Hong Liou   Group: iGEM11_NYMU-Taipei   (2011-10-05)

revised minC primer (reverse)

As mentioned in the description of BBa_K624035, this was the reverse primer we used.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
    Illegal NotI site found at 10
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 19
    Illegal PstI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/arevised minC primer (reverse)