Generator

Part:BBa_K624023

Designed by: Shang-Jui Tsai   Group: iGEM11_NYMU-Taipei   (2011-10-03)

pYMB essentials+[Y-13-R]

Positive control for BRET. This also serve to examine the effects of N-terminal fusion on correct folding of mms13 (the "13" indicated in the name).

Fig. 6: This design is constructed with the whole YFP on the N-terminus of Mms13 but “without” the CHAMP design. The construct should be a positive control with constant light no matter whether force is applied or not.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 2034
    Illegal NgoMIV site found at 2118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2784


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