Part:BBa_K622000

rpoN: coding sigma 54 factor

This part codes sigma 54 factor of E.coli and its RBS. We use this part to make nutritional switch which is activated under poor availability of combined nitrogen. The complex of sigma 54 factor and the other transcription factor activates sigma 54 promoter.

Usage and Biology

We are Team:IIT-Madras 2015, we are using this Biobrick for our project with new application. Sigma-54 plays a role in quorum sensing of Vibrio harveyi, which uses AutoInducer-2(AI-2) as the quorum sensing molecule. At conditions of low cell density(when AI-2 is low), LuxQ with the periplasmic protein LuxP transfer phosphate to LuxO. LuxOP in conjunction with Sigma54 indirectly represses expression of the genes encoding luciferase (lux), so no light is produced under this condition. At low cell density, LuxO∼P, together with 􏰀Sigma54, activates the expression of the genes encoding four small regulatory RNAs (sRNAs) called Qrr1-4(Quorum regulatory RNA's).
Reference : Kimberly C. Tu and Bonnie L. Bassler. Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi. GENES & DEVELOPMENT 21:221–233.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 591
  Illegal BamHI site found at 554
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 463
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 250