Reporter

Part:BBa_K619896

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-27)


Pcon lox gent double terminator lox

This construct contains a constitutive promoter (BBa_J23106) followed by gentamycin resistance surrounded by lox sites. This part will allow expression from a downstream reporter from the constitutive promoter when Cre is expressed. Thus, one can initiated expression of Cre from an inducible promoter, and have constitutive expression of a reporter and loss of gentamycin resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 811
    Illegal BamHI site found at 1300
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167


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Categories
Parameters
None