Regulatory

Part:BBa_K619895

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-27)


mutated prfA-UTR thermosensor from Listeria monocytogenes

This is the prfA-UTR thermosensor mutated to produce a thermosensor with increassed expression at both 30 and 37 degrees. On can still detect an increase in expression when switching from 30 to 37 degrees. Characterization of its dynamic range is underway. The part was made by TAQ-mediated random mutagenesis of our "wild-type" thermosensor (BBa-19889) and subsequent screening using a lacZ reporter. There are other thermosensors entered into the IGEM database with different dynamic ranges obtained from this screen. To use this thermosensor, be sure to clone in frame with the indicated initiating ATG.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None