Regulatory

Part:BBa_K619889

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-26)


prfA-UTR, RNA thermosensor from Listeria monocytogenes

This part contains prfA-UTR, an RNA thermosensor from Listeria monocytogenes originally characterized by Johansson et al. Cell 110:551561. The secondary structure of this RNA forms at hairpin at 30'C which masks the ribosomal binding site and initiating ATG. At 37'C this hairpin melts allowing ribosomes to bind and translation to proceed. Be sure that your gene fusion is in frame since the initiating ATG for translation is encoded in this DNA (see diagram from paper). Sequence just before initiating ATG is GAGACATG where the ATG in this sequence is the initiating ATG.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None