Composite

Part:BBa_K618212

Designed by: Ben Park, Chris Bate, Marc Ammerlaan   Group: iGEM11_Michigan   (2011-09-27)


pLAC ompA-zif268

This is a fusion between ompA and the DNA binding protein zif268 under the control of pLAC. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 773
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane/extracellular
//chassis/prokaryote/ecoli
//proteindomain/dnabinding
Parameters
None