Composite

Part:BBa_K618112

Designed by: Chris Bate, Ben Park, Marc Ammerlaan   Group: iGEM11_Michigan   (2011-09-27)


pBAD ompA-zif268

This is a fusion between ompA and the DNA binding protein zif268 under the control of pBAD. The protein should be present on the outer surface of cells and bind the zif268 recognition sequence (GATGCTGCA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
    Illegal AgeI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107


[edit]
Categories
//cds/membrane/extracellular
//chassis/prokaryote/ecoli
//proteindomain/dnabinding
Parameters
None