Composite

Part:BBa_K618111

Designed by: Chris Bate, Ben Park, Marc Ammerlaan   Group: iGEM11_Michigan   (2011-09-27)

pBAD ompA-Gli1

This is a fusion between ompA and the DNA binding protein Gli-1 under the control of pBAD. The protein should be present on the outer surface of cells and bind the Gli-1 recognition sequence (GACCACCCAAGACGA). This will also allow cells to be anchored to a glass slide with the appropriate DNA attached. This can serve as a way to position cells in any desired pattern.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 107


[edit]
Categories
//cds/membrane/extracellular
//chassis/prokaryote/ecoli
//proteindomain/dnabinding
Parameters
None