Plasmid

Part:BBa_K617000

Designed by: Will Jones   Group: iGEM11_UIUC-Illinois   (2011-09-27)


Biobrick Compatible Lambda Chromosomal Insertion Plasmid

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2398
    Illegal suffix found in sequence at 2420
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2398
    Illegal NheI site found at 273
    Illegal SpeI site found at 2421
    Illegal PstI site found at 2435
    Illegal NotI site found at 1102
    Illegal NotI site found at 2404
    Illegal NotI site found at 2428
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2398
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2398
    Illegal suffix found in sequence at 2421
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2398
    Illegal XbaI site found at 2413
    Illegal SpeI site found at 2421
    Illegal PstI site found at 2435
    Illegal NgoMIV site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2129
    Illegal SapI site found at 1258
    Illegal SapI site found at 1468


[edit]
Categories
//function/recombination/lambda
Parameters
None