Measurement

Part:BBa_K611083

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-08-21)


BBa_C0051 Test Construct +1(TSS) delete

This part is identical to the BBa_C0051 Test Construct (BBa_K611061) except the transcriptional start site has been deleted. This is a thymine base immediately following the barcode and immediately preceding the SpeI site.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2926
    Illegal XbaI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2926
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal NotI site found at 877
    Illegal NotI site found at 2932
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2926
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2926
    Illegal XbaI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2926
    Illegal XbaI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 884
    Illegal AgeI site found at 580
    Illegal AgeI site found at 692
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1965


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