Measurement

Part:BBa_K611082

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-08-17)


GFP (BBa_E0040) Screening Plasmid

This part is a GFP version of the BBa_J61002 screening. All elements are the same except RFP has been replaced with GFP. This part was made in order to characterize the promoter activity in the LVA-TAATAA-Barcode region of BBa_C0051.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2941
    Illegal XbaI site found at 2956
    Illegal SpeI site found at 1
    Illegal PstI site found at 899
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2941
    Illegal SpeI site found at 1
    Illegal PstI site found at 899
    Illegal NotI site found at 892
    Illegal NotI site found at 2947
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2941
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2941
    Illegal XbaI site found at 2956
    Illegal SpeI site found at 1
    Illegal PstI site found at 899
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2941
    Illegal XbaI site found at 2956
    Illegal SpeI site found at 1
    Illegal PstI site found at 899
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 669
    Illegal BsaI.rc site found at 1980


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