Part:BBa_K611080

LVA tag, Double Stop Codon, Barcode test construct

This part is the test construct of BBa_K611103 used for pinpointing the location of promoter activity in the LVA-TAATAA-Barcode region of BBa_C0051.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2927
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  Illegal NotI site found at 878
  Illegal NotI site found at 2933
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2927
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2927
  Illegal XbaI site found at 2942
  Illegal SpeI site found at 2
  Illegal PstI site found at 885
  Illegal AgeI site found at 581
  Illegal AgeI site found at 693
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1966