Measurement

Part:BBa_K611078

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-05-17)


LVA 5' -11bp, Barcode 3' -16bp Truncation (BBa_K611101) test construct

This part is the test construct of BBa_K611101 used for pinpointing the location of promoter activity in the LVA-TAATAA-Barcode region of BBa_C0051. This construct contains the LVA tag truncation where the first 11 bases on the 5' end have been truncated as well as the last 16 bases of the Barcode on the 3' end.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2927
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1966


[edit]
Categories
Parameters
None