Measurement

Part:BBa_K611077

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-05-17)

LVA 5' -22bp, Barcode 3' -8bp Truncation (BBa_K611100) test construct

This part is the test construct of BBa_K611100 used for pinpointing the location of promoter activity in the LVA-TAATAA-Barcode region of BBa_C0051. This construct contains the LVA tag truncation where the first 22 bases on the 5' end have been truncated as well as the last 8 bases of the Barcode on the 3' end.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2927
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1966


[edit]
Categories
Parameters
None