Measurement

Part:BBa_K611068

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-05-16)


Truncation C test construct

This construct is used for testing the promoter activity in the barcode region of the cI Lambda biobrick, (BBa_C0051). The first 260 bases have been truncated in order to rule out the possibility that leaky expression of the protein was affecting the transcription of the downstream RFP (BBa_E1010).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2927
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1966


[edit]
Categories
Parameters
n/aTruncation C test construct