Measurement

Part:BBa_K611062

Designed by: Timothy Fenton   Group: iGEM11_UC_Davis   (2012-05-15)


BBa_B0034-BBa_E1010 Test Construct in BBa_J61002

This part is used for testing the promoter activity in the barcode region of BBa_E1010 when placed upstream of another RBS (BBa_B0034) and RFP (BBa_E1010). BBa_I13502 was ligated into the BBa_J61002 plasmid to create this construct.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2927
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 2933
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2927
    Illegal XbaI site found at 2942
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
    Illegal AgeI site found at 3529
    Illegal AgeI site found at 3641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1966


[edit]
Categories
Parameters
n/aBBa_B0034-BBa_E1010 Test Construct in BBa_J61002