Composite

Part:BBa_K611033

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-27)


LacI Promoter Variant #3 in Characterization Construct

This part contains a variant of the wild type LacI repressible promoter, and is followed by the LacI characterization construct, K611013. This allows for testing of this particular mutant promoter at various concentrations of repressor. The mutated LacI promoter in this plasmid expresses GFP and is followed by an arabinose inducible promoter, pBad, which expresses the LacI repressor. Thus, a user can increase the concentration of repressor by inducing pBad with arabinose. Similarly, the repressor may be unrepressed with the addition of IPTG. This plasmid is very useful for the further characterization of K611023.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2394
    Illegal BamHI site found at 1156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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