Composite

Part:BBa_K611016

Designed by: Nicholas Csicsery   Group: iGEM11_UC_Davis   (2011-09-18)


LacI Repressor and Promoter Wild Type Control

This part is can be used as a control for BBa_K611010 and BBa_K611013, which test either C0012 and R0010 mutants respectively.

For 2017, Team UAlberta submitted BBa_K2389070, which replaces the arabinose-regulated promoter for LacI was with a constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2394
    Illegal BamHI site found at 1156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870


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