Composite

Part:BBa_K609005

Designed by: Ke Liu   Group: iGEM11_Harbin_Inst_of_Tech_Food_   (2011-10-02)


human collagen type Ⅲ+ nisI (without promoter)

It is the combination of a section of human collagen gene with the Nisin resistance gene nisI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 514
    Illegal EcoRI site found at 559
    Illegal XbaI site found at 574
    Illegal SpeI site found at 1479
    Illegal PstI site found at 1493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 559
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1479
    Illegal PstI site found at 529
    Illegal PstI site found at 1493
    Illegal NotI site found at 15
    Illegal NotI site found at 522
    Illegal NotI site found at 565
    Illegal NotI site found at 1486
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 559
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 515
    Illegal EcoRI site found at 559
    Illegal XbaI site found at 574
    Illegal SpeI site found at 1479
    Illegal PstI site found at 1493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal EcoRI site found at 559
    Illegal XbaI site found at 24
    Illegal XbaI site found at 574
    Illegal SpeI site found at 515
    Illegal SpeI site found at 1479
    Illegal PstI site found at 529
    Illegal PstI site found at 1493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1106


[edit]
Categories
Parameters
None