Composite

Part:BBa_K609001

Designed by: Ke Liu   Group: iGEM11_HIT-Harbin   (2011-10-01)


acid induced promoter rcfB + repressor lacR of lac operon


When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB, will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1112
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 1113
    Illegal PstI site found at 1127
    Illegal NotI site found at 15
    Illegal NotI site found at 1120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BglII site found at 222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 1113
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 1113
    Illegal PstI site found at 1127
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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