Part:BBa_K609000

nisI+pH induced promoter rcfB+ repressor LacR of lac operon

When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB, will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.And the nisI gene is a Nisin resistance gene which could be used in food.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 9
  Illegal suffix found in sequence at 841
  Illegal EcoRI site found at 886
  Illegal XbaI site found at 901
  Illegal SpeI site found at 1990
  Illegal PstI site found at 2004
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 9
  Illegal EcoRI site found at 886
  Illegal SpeI site found at 842
  Illegal SpeI site found at 1990
  Illegal PstI site found at 856
  Illegal PstI site found at 2004
  Illegal NotI site found at 15
  Illegal NotI site found at 849
  Illegal NotI site found at 892
  Illegal NotI site found at 1997
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 9
  Illegal EcoRI site found at 886
  Illegal BglII site found at 1099
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 9
  Illegal suffix found in sequence at 842
  Illegal EcoRI site found at 886
  Illegal XbaI site found at 901
  Illegal SpeI site found at 1990
  Illegal PstI site found at 2004
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 9
  Illegal EcoRI site found at 886
  Illegal XbaI site found at 24
  Illegal XbaI site found at 901
  Illegal SpeI site found at 842
  Illegal SpeI site found at 1990
  Illegal PstI site found at 856
  Illegal PstI site found at 2004
  
• 1000
  COMPATIBLE WITH RFC[1000]