Translational_Unit

Part:BBa_K608151

Designed by: Julia Müller   Group: iGEM11_Freiburg   (2011-09-14)

translational unit of pcyA

scheme of enzyme ho1 and pcyA involved in chromophore production

We designed this composite in order to gain the enzyme pcyA (phycocyanobilin-ferredoxin oxidoreductase),
this design is necessary if the part is going be integrated into an assembly of various genes.
The enzyme plays an important role in the production of the PCB chromophore (phycocyanobilin)
which is essential for the green and red light receptor system.
Our sequencing confirmed that the part is correct in the pSB1C3 vector.
All physical DNA samples used for this composite come from the registry distribution.


Usage and Biology

For PCB chromophore production the enzyme ho1 (heme oxygenase)
in a similar design is necessary infront of this part
because both enzymes are needed to convert heme into PCB chromophore.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 830
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
  • 1000
    COMPATIBLE WITH RFC[1000]


references

Ken Okada "HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis"
FEBS letters, Volume 583, Issue 8, 17 April 2009, Pages 1251-1256

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