Composite

Part:BBa_K607034

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-18)

PlasB_LasR_antipBad/araC with 1.0RBS

This part is a member of LasR autoinducing loop family.

LasR protein without degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.

Caption test Lciaulegend.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
    Illegal NheI site found at 1052
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 642
    Illegal AgeI site found at 839
    Illegal AgeI site found at 1278
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1295


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Parameters
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