Part:BBa_K607015
cI
cI without degradation tag
The cI gene codes for the bacteriophage λ repressor(1). This bacteriophage λ-repressor from phage lambda does not contain a ssrA tag. The ssrA tag is a target for proteolytic enzymes and causes degradation of the protein(2). When this gene is transcribed, it will not be degraded that fast by proteolytic enzymes ( because it lacks the ssrA tag) and so the protein half-life will be longer compared to cI with a degradation tag.
References:
(1)Robert T. Sauer* and Robert Anderegg. Primary Structure of the λ Repressor. Biochemistry, 1978.
(2) Jeff Hasty, David McMillen, Farren Isaacs and James J. Collins. Computational studies of gene regulatory networks: in numero molecular biology. Nature 268
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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