Composite

Part:BBa_K607012

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-18)

Prm_cI-LVA_antiPlasB with 0.6RBS

This part is a member of Lambda cI autoinducing loop family.

cI with LVA-tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.6 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))

Caption test Lciaulegend.png

Figure 1. This autoinducing loop does not exhibit heterogenity when non-induced. Only the population that is not producing GFP is observed. That means that the loop is not induced through a leaky promoter and is a good candidate for an inducible bistable switch.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1126
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aPrm_cI-LVA_antiPlasB with 0.6RBS