Composite

Part:BBa_K607005

Designed by: Olesja Popow   Group: iGEM11_Groningen   (2011-09-14)

Prm_crRNA_LasR-LVA

crRNA coupled LasR with LVA-tag under transcriptional control of Prm promoter.
The crRNA is composed of the sequence of the target gene and an upstream short nucleotide sequence which is complementary to the ribosome binding site (RBS). Repression of gene expression is achieved through sequestration of the RBS by formation of a stem loop structure by the 5'-untranslated region of the crRNA. This prevents binding of the RBS by the 30s ribosomal subunit and subsequent translation.
For more information about the LasR-LVA, see https://parts.igem.org/wiki/index.php?title=Part:BBa_K607002
For more information about the Prm promotor, see: https://parts.igem.org/wiki/index.phptitle=Part:BBa_K607003title=Part:BBa_K607003
References:
Isaacs at al. 2004



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 458
    Illegal AgeI site found at 655
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None