Generator

Part:BBa_K606067

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-10-23)


Upstream LacI expression IPTG inducible tRNA emitter with RFP Monitor

This device are the emitter system for our tRNA diffusion system monitored by RFP fluorescence. It express the tRNA amber who is needed for expression of T7 Amber. In addition a strong LacI expression cassette is added to repress pHyperspank because B. subtilis doesn't express LacI constitutively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2032
    Illegal AgeI site found at 2144
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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