Generator
LacI-pHS-t

Part:BBa_K606066

Designed by: Kvin Yauy   Group: iGEM11_Paris_Bettencourt   (2011-10-23)


Upstream LacI expression IPTG inducible tRNA emitter

This device are the emitter system for our tRNA diffusion system. It express the tRNA amber who is needed for expression of T7 Amber. In addition a strong LacI expression cassette is added to repress pHyperspank because B. subtilis doesn't express LacI constitutively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None