SpoVG Ribosome Binding Site (RBS) for B. subtilis
SpoVG Ribosome Binding Site (RBS) for B. subtilis.
This is a strong ribosome binding site from suibtilis. This part is a copy of the part from Imperial college 2008, BBa_K143021, but we have synthetized it de-novo, using primer matching technique, and submitted it to the registry.
This parts also works fine in E. coli.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
Usage and Biology
This part can be use to express proteins in both B. subtilis and E. coli, and leads to a strong expression of the protein. The original sequence come from the reference following reference 
- 1 pmid=9669336 [Mol Gen Genet. 1998 Jun;258(5):538-45]