T7

Part:BBa_K606056

Designed by: Axel Séguret   Group: iGEM11_Paris_Bettencourt   (2011-09-18)

Double terminator + Lambda phage attR recombination site

Lambda phage attR recombination site from the lambda phage cloned with a double terminator on the left. It has been synthetized de novo by GeneArt and submitted to the registry once clones into pSB1C3.

The idea is that this part can be used in combination with the part K606049. When expressing the Xis protein, the stop can be excised, and the transcription recovered.

Excision xis-reporter.jpg

The sequence of this part is not exactly the one you can find for the similar parts in the registry. We took it from cross-validating several articles from the litterature.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None