Generator

Part:BBa_K606039

Designed by: Axel Séguret   Group: iGEM11_Paris_Bettencourt   (2011-09-18)

pVeg SpoVG T7 SpoVG RFP double terminator.

T7 and RFP expression system in B. subtilis. The RFP is on the same mRNA in order to have a RFP signal proportionnal to the quantity of T7 RNA polymerase produced. There is probably a ration 1:1 at the stationnay phase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3359
    Illegal AgeI site found at 3471
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/positive
Parameters
None