Deleted
Sample Discontinued
Experience: None
Not Used
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Translational_Unit

Part:BBa_K606015

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-07-07)


T7 GFP expression system for B. Subtilis

This is a GFP expression system for B. Subtilis that is dont to be polymerazed by the T7 polymerase. This have to be activated by the pT7 promoter I712074. The RBS used is SpoVG, that is design for a strong expression in B. Subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662


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Parameters
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